Construction of the bsl 3 laboratory until recently, the bsl 3 laboratory has exclusively been constructed as a traditional fixed site facility. Bsl3 facility planning issues design concepts cell culture rooms air lock accessioning decon bsl3 equipment sample prep. Bsl3 labs are also used for handling pathogens with a higherrisk or unknown risk, especially when the facility is outfitted with a class iii biosafety cabinet. The rbls are required to have bsl 3 and bsl 2 labs, animal facilities, and research support space. Designing and operating a bsl3 facility traum institute. Several years ago, president napolitano formed the uc highcontainment laboratory oversight. Biosafety level 3 bsl3 is a designation assigned by. The rbls are required to have bsl3 and bsl2 labs, animal facilities, and research support space. Concurrently, there has been an increase in demand for cell sorting in bsl3 laboratories and therefore requirements to conduct these experiments safely. The laboratory should be designed so that it can be easily cleaned and decontaminated. The bsl 3 facility design, operational parameters and procedures must be verified and documented prior to operation.
In addition to these criteria, any bsl3 shall conform to the latest edition of biosafety in microbiologic and biomedical laboratories bmbl in effect at the start of. The design must be closely studied and evaluated for safety, capital and operating cost, maintenance, accessibility, construction and validation schedule goals, as well as any additional client requirements. Flexibility may be more readily achieved by including several levels of containment in one facility, such as bsl3, absl3, and bsl3ag. What bsl3 containment facilities does the university have. Each model should be used based on the program to be housed and its specific requirements. Many structured opportunities to network and share challenges and solutions with instructors and fellow students. Designing hvac systems for biosafety level requirements. Doors should be selfclosing and lockable in order to restrict access to the laboratory.
Work at bsl3 requires enhanced facility design, operational controls and special practices, which will be. The biosafety manual is prepared or adopted by the laboratory director, and biosafety precautions are incorporated into the labs standard operating procedures. Biosafety level 3 bl3 ragon institute of mgh, mit and harvard. However, if a bsl3 laboratory is within a larger building, the actual laboratory rooms and associated support rooms should be separated from the other areas of the building. In addition to initial hvac verification and reverification as described above, the following are the minimum facility verification requirements that an entity is expected to perform and document initially for a. Biosafety level 3 bsl 3 agents are those requiring containment of airborne bacteria, viruses, or toxins, therefore the design and administration of the bsl3 biocontainment facility follows strict federal regulations with local oversight provided by the ncsu institutional. Based approach in designing and operating a bsl3 facility. Therefore, usda20 defined bsl 3 ag criteria enhances containment described for bsl 3 by adding20 filtration of supply and exhaust air, sewage decontamination, exit personnel20 showers, and facility integrity testing. Researchers perform all experiments in a biosafety cabinet. A bsl3 should be designed to allow it to perform independently of other building systems. Three ibcs with bsl 3 and 1 with bsl 3 and bsl 4 facilities elected not to participate.
Specific education and training programs for laboratory workers together with the design and construction of proper laboratory facilities must be followed to evade. Carpets and rugs in laboratories are not appropriate. The bsl 4 laboratory should contain a class iii biological safety cabinet but may use a class i or ii bsc in combination with a positivepressure, airsupplied fullbody suit. A bsl3 laboratory has special engineering and design features, and a biosafety manual specific to the laboratory and organisms in use. Over the past decade, there has been a rapid growth in the number of bsl 3 and bsl 4 laboratories in the usa. Concurrently, there has been an increase in demand for cell sorting in bsl 3 laboratories and therefore requirements to conduct these experiments safely. Nbbtp is offering bsl 3 operations and maintenance ii, a fiveday highcontainment laboratory training course designed for facilities personnel, laboratory managers, biosafety, design and engineering professionals. Germfree integrated modular offers significant advantages over sitebuilt construction.
Installation of bsl3 laboratories and absl3 animal experimentation rooms in a preexisting bsl3ag facility. Design of bsl3 laboratories chapter 7 sage journals. Whether youre in the office or on the go, youll enjoy the full set of features, symbols, and highquality output you get only with smartdraw. Your feedback and questions on this manual are very important to us. Detailed statistics on bsl4 safety in the united states can be found in a recent report. Biosafety level 3 program ahc research services university of. Facility design considerations for select agent animal. Therefore, usda20 defined bsl3ag criteria enhances containment described for bsl3 by adding20 filtration of supply and exhaust air, sewage decontamination, exit personnel20 showers, and facility integrity testing. The laboratory should be designed so that it can be easily cleaned. A the purpose of this guideline is to establish the criteria for design and construction of level 3 biological safety laboratories bsl3 at uncchapel hill. This hierarchy of containment typically results in a project having a lower construction cost than a comparably sized project dedicated only to bsl3ag. In a subsequent project, the work flow was altered to provide for bsl3 animal holding areas. Bsl3 design guidelines 2010 design guidelines 4 p a g e 2 cabinets must comply with and be installed and certified in accordance with nsfansi 492002 3 if a cabinet contains both a down flow fan and an exhaust fan, the fans shall be interlocked so that the down flow fan shuts off whenever the exhaust fan fails. Bsl3 facilities must be absl3 facilities should be verified at least annually to show that operational parameters can be met.
The levels of containment range from the lowest biosafety level 1 bsl 1 to the highest at level 4 bsl 4. Powerful plant layout and facility planning tools no matter how complex your plan, smartdraw will help you align and arrange all your elements perfectly. Bsl3 biocontainment facility consists of two large laboratories each with 3 work spaces built around biosafety cabinets. Apr 28, 2020 a mix of case studies, lecture, demonstrations, facility tours, group exercises and handson workshops. Pathogens and bsl3 laboratory there are about 10 million microbe species have been identified in the world. Integrated modular facilities bsl3 laboratories germfree. Federal select agent program bsl3absl3 verification. These alterations to the facility and exhaust system provided necessary redundancy at a cost savings. Biosafety level 3 bl3 the bl3 core provides training and consultation for members of the hu cfar community who are planning tb related projects and provides access to bsl3 tissue culture, live cell sorting, and widefield live cell videomicroscopy.
Facilities must be reverified and documented at least annually. Preliminary authorization basis documentation for the. Facilities ahc research services university of minnesota. This highly innovative approach provides new laboratory space that is engineered for your specific technical and architectural requirements. The receiving scientist must be added as the responsible party in the pathogen database and all required records must be updated to document such. Examples of microbes worked with in a bsl 3 includes. The remaining 381 contacts were sent a reminder email 2 weeks before the close of the survey. Unc chapel hill design requirements for level 3 biological. Based approach in designing and operating a bsl 3 facility. Concentrate minimize bsl3 and 4 limit number of individuals and processes that must work under bsl3 and bsl4 conditions provide appropriate service support outside containment zone provide adequate functional support inside containment zone estimate capital and. Policy statement, bsl 3 absl 3 facility verification. The 5day bsl 3 operations and maintenance ii training program is designed for facilities personnel, laboratory managers, and biosafety professionals. Plant layout and facility software free online app. Basic laboratory design for biosafety level 3 laboratories.
Bsl3 laboratory and associated support rooms are in an entirely separate building. Bsl3 labs are unique facilities with complex design, mechanical and engineering features to keep the facility under negative air pressure at all times. The previous wusm laboratory design guidelines combined bsl2, bsl3 and absl3 into one category of high hazard. Design, implementation, validation, time requirements, and costs urs pauli, martin kundig, fritz haldemann, and kathrin summermatter. More protective primary barriers are used in bsl 3 laboratories, including solidfront wraparound gowns, scrub suits or coveralls made of materials such as tyvek and respirators as necessary. Flexibility may be more readily achieved by including several levels of containment in one facility, such as bsl 3, absl 3, and bsl 3ag. The bsl3 facility design, operational parameters and procedures must be verified and documented prior to operation. In order for a bsl3 absl3 hvac to meet this requirement, initial hvac design verification must be performed and documented by someone with experience and expertise with the hvac system prior to operation. Feb 01, 2014 at 36 facilities, multiple ibcs were affiliated with the same facility. Biosafety level 3 bsl 3 bsl 3 laboratories are used to study infectious agents or toxins that may be transmitted through the air and cause potentially lethal infections. Pdf establishment of biosafety level3 bsl3 laboratory. This initial hvac design verification ensures that secondary.
A mix of case studies, lecture, demonstrations, facility tours, group exercises and handson workshops. Using a riskbased approach can save a bsl3 facility tremendous dollars for operation, maintenance and construction. The absl3 facility design and operational procedures must be documented. Bsl 3 absl 3 research laboratory suites a 2,900 squarefeet, fully equipped biosafety level 3 bsl 3 research space on the minneapolis campus is available to scientists at the university of minnesota or their research partners for studies involving risk group 3 agents and animal research. They will learn how to develop operational procedures to manage safety, security and compliance, and put in place a robust safety system for their facility. Federation of american scientists us bsl laboratories. Bsl3absl3 research laboratory suites a 2,900 squarefeet, fully equipped biosafety level 3 bsl3 research space on the minneapolis campus is available to scientists at the university of minnesota or their research partners for studies involving risk group 3 agents and animal research. Bsl3 operations and maintenance ii may 2018 description.
The following bsl3 design guidelines are intended to assist the washington university school of. Ingenium building services limited mechanical building. Intuitive planning interface you save timenow you can quickly plan your facility plan without having to learn difficult architectural software. In the united states, the centers for disease control and prevention cdc have specified these levels. Ingenium provided mechanical design and bim services to parsons brinckerhoff on the conference facility within the new build spire hospital in manchester. Facility design should incorporate selfclosing doubledoor access separated from general building corridors.
Three ibcs with bsl3 and 1 with bsl3 and bsl4 facilities elected not to participate. Separation methods can include locating the bsl 3 laboratory unit or suite. This containment facility includes three standalone research suites. Again building upon the two prior biosafety levels, a bsl 3 laboratory typically includes work on microbes that are either indigenous or exotic, and can cause serious or potentially lethal disease through inhalation. Laboratory furniture must be capable of supporting anticipated loads and uses.
In addition to these criteria, any bsl3 shall conform to the latest edition of biosafety in microbiologic and biomedical laboratories bmbl in effect at the start of construction. Construction of the bsl3 laboratory until recently, the bsl3 laboratory has exclusively been constructed as a traditional fixed site facility. The biosafety officer in collaboration with the institutional biosafety committee must approve the location and design of any bsl3 facility, and has final. In a subsequent project, the work flow was altered to provide for bsl 3 animal holding areas. The room pressurization was adjusted and redundant exhaust fans added and relocated on the roof. The facility must be tested to verify that the design and. Installation of bsl3 laboratories and absl3 animal.
Biosafety level 3 bl3 ragon institute of mgh, mit and. E the brand, generation, and software tools for the bsl3 bas shall be the same as that used. Bsl 3 labs are also used for handling pathogens with a higherrisk or unknown risk, especially when the facility is outfitted with a class iii biosafety cabinet. Biosafety level 3 bsl3 laboratory design standards ucop. Bsl 3 laboratory and associated support rooms are in an entirely separate building. The levels of containment range from the lowest biosafety level 1 bsl1 to the highest at level 4 bsl4. To the bsl 3 practices, we add requirements for complete clothing change before entry, a shower on exit and decontamination of all materials prior to leaving the facility.
You save timenow you can quickly plan your facility plan without having to learn difficult architectural software. Solid easily cleanable surfaces floor, walls, ceiling c. This requires complex hvac systems to maintain directional airflow, anterooms airlocks and interlocking doors. An occupational health program exists for medical surveillance of laboratory workers. Biosafety level 3 bsl3cl3 laboratory solutions the celeris critical environmental control platform from phoenix controls is designed to provide unmatched airflow and space comfort control for todays demanding highlevel containment level 3 bsl3cl3 facilities. Special engineering and design features are built into these facilities, including the ability to. Engineering controls, including facility construction and design elements in. The design of hvac systems to meet biosafety level requirements presents special challenges. Separation methods can include locating the bsl3 laboratory unit or suite. Laboratory biosafety describes the application of specific practices, safety. Carpets and rugs in laboratories are not permitted. The final pool was 359 potential bsl 3 respondents. Design engineers, facility managers, architects, commissioning agents, and anyone involved in designing, managing or maintaining a bsl3. This hierarchy of containment typically results in a project having a lower construction cost than a comparably sized project dedicated only to bsl 3ag.
In addition to initial hvac verification and reverification as described above, the following are the minimum facility verification requirements that an entity is expected to perform and document initially for a bsl 3 or absl 3 laboratory and again at least annually. Create facility plans from any devicewindows, mac, android, ios, or any other platform with an internet connection. Using a riskbased approach can save a bsl 3 facility tremendous dollars for operation, maintenance and construction. Vivarium vivarium support bsl 3 bsl 3 holding bsl 3 support bsl3 barrier. Biosafety level 3 bsl3 agents are those requiring containment of airborne bacteria, viruses, or toxins, therefore the design and administration of the bsl3 biocontainment facility follows strict federal regulations with local oversight provided by the ncsu institutional. Basic laboratory design for biosafety levels 1 and 2 lab. A bsl3 pathogen can be transferred internally to another scientist within the same facility, providing that the biosafety level for containment and the level of staff competence are maintained.
Science safety security finding the balance together. Basic laboratory design for biosafety levels 1 and 2upbasic laboratory design for biosafety level 3. At 36 facilities, multiple ibcs were affiliated with the same facility. The purpose of this guideline is to establish the criteria for design and construction of level 3 biological safety laboratories bsl3 at uncchapel hill. Absl3 and bsl3 facilities shall be designed such that under failure conditions the airflow will not be reversed. The sandwich structure of a bsl3 lab bsl3 lab design requirements. A biosafety level bsl, or pathogenprotection level, is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility.
Standard practice for cell sorting in a bsl3 facility. Over the past decade, there has been a rapid growth in the number of bsl3 and bsl4 laboratories in the usa. However, if a bsl 3 laboratory is within a larger building, the actual laboratory rooms and associated support rooms should be separated from the other areas of the building. In addition to these criteria, any bsl3 shall conform to the latest edition of biosafety in microbiologic and biomedical laboratories bmbl in effect at. A 2,900 squarefeet, fully equipped bsl3 research facility on the minneapolis campus is available to scientists at the university of minnesota or their research partners for studies involving bsl3 agents and animal research. Current training programs biosafety training program. No matter how complex your plan, smartdraw will help you align and arrange all your elements perfectly. A steam autoclave was added within the bsl 3 suite. While fulfilling the need of researchers occupying the facility, the nbls and rbls can be used by other biodefense researchers within the region, particularly those within the regional centers of excellence in biodefense and emerging infectious. A 2,900 squarefeet, fully equipped bsl 3 research facility on the minneapolis campus is available to scientists at the university of minnesota or their research partners for studies involving bsl 3 agents and animal research. Bsl 3 labs are unique facilities with complex design, mechanical and engineering features to keep the facility under negative air pressure at all times.